產(chǎn)品屬性：
產(chǎn)品名稱 | Ethidium bromide |
規(guī)格 | 10mM*1 mL in DMSO、250mg |
貨號 | EY-01Y19839 |
Cas No.: 1239-45-8
別名: N/A
化學(xué)名: N/A
分子式: C21H20BrN3
分子量: 394.31
溶解度: H2O: ≥ 100 mg/mL (253.61 mM); DMSO: 83.33 mg/mL (211.33 mM)
儲存條件: Store at RT
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
產(chǎn)品描述：
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).The preparation of agarose gel:1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.2. Mix the agarose powder with 1X TAE/TBE.3. Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil).4. Make the solution cool down before solidification.5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 μL stock/100 mL gel).6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection. [1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.
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