鑻辨枃鍚嶇ū(ch膿ng)锛欰mycolata autotrophicaPCR
"/>
鏀惰棌鏈珯
鎴戠殑璩囨枡
鎴戠殑瑷傚柈
  璩�(g貌u)鐗╄粖(ch膿) (0)  
瑕紝鎮ㄧ殑璩�(g貌u)鐗╄粖(ch膿)绌虹┖鐨勫柌~
鍘昏臣(g貌u)鐗╄粖(ch膿)绲�(ji茅)绠�
   
鏌ョ湅鎵嬫(j墨)缍�(w菐ng)绔�
鍏朵粬璩櫉(h脿o)鐧婚寗锛� 娉ㄥ唺(c猫) 鐧婚寗
150-21460884
鐢�(ch菐n)鍝佸垎椤�(l猫i)
鑷(y菐ng)鐒�(w煤)鏋濋吀鑿孭CR妾㈡脯(c猫)瑭﹀姂鐩� 
鑷(y菐ng)鐒�(w煤)鏋濋吀鑿孭CR妾㈡脯(c猫)瑭﹀姂鐩�
鏀惰棌
|
|
鑻辨枃鍚嶇ū(ch膿ng) 锛� Amycolata autotrophicaPCR
璨ㄨ櫉(h脿o) 锛� EY00P74
瑕�(gu墨)鏍� 锛� 50T
鍝佺墝 锛� 涓婃捣涓€鐮�
鍍�(ji脿)鏍� 锛�
锟�0.00
璩�(g貌u)璨�(m菐i)鏁�(sh霉)閲忥細
鍔犲叆璩�(g貌u)鐗╄粖(ch膿)  绔嬪嵆璩�(g貌u)璨�(m菐i)
鐢�(ch菐n)鍝佷繚璀�
姝e搧淇濊瓑
蹇€熺櫦(f膩)璨�
鐢�(ch菐n)鍝佽┏鎯�
鐢�(ch菐n)鍝佽⿻(p铆ng)璜�(0)
閵�(xi膩o)鍞閷�(0)

瑷傝臣(g貌u)淇℃伅锛�

鐢�(ch菐n)鍝佸悕绋�(ch膿ng)鑷(y菐ng)鐒�(w煤)鏋濋吀鑿孭CR妾㈡脯(c猫)瑭﹀姂鐩�
鑻辨枃鍚嶇ū(ch膿ng)Amycolata autotrophicaPCR
椤�(l猫i)鍒�PCR妾㈡脯(c猫)瑭﹀姂鐩�

鐢�(ch菐n)鍝佸悕绋�(ch膿ng)锛�鑷(y菐ng)鐒�(w煤)鏋濋吀鑿孭CR妾㈡脯(c猫)瑭﹀姂鐩�

鑻辨枃鍚嶇ū(ch膿ng)锛欰mycolata autotrophicaPCR

鍒嗛(l猫i)锛歅CR妾㈡脯(c猫)瑭﹀姂鐩�

鍎�(ch菙)瀛樻浠讹細-20鈩冮伩鍏変繚瀛橈紝閬垮厤鍙嶅京(f霉)鍑嶈瀺銆�

閬�(y霉n)杓革細浣庢韩銆侀伩鍏夛紝蹇仦鍏嶈不(f猫i)閫佽波涓婇杸(m茅n)銆�

鐗归粸(di菐n)锛�

1.鍗抽枊(k膩i)鍗崇敤锛岀敤鎴�(h霉)鍙渶瑕佹彁渚涙ǎ鍝� DNA 妯℃澘锛屾搷浣滅啊(ji菐n)鍠�锛屽畾閲忔簴(zh菙n)纰哄揩閫�銆�

2. 寮曠墿缍�(j墨ng)閬�(gu貌)鍎�(y艒u)鍖�锛岀壒鐣版€у挤(qi谩ng)銆傞爯(y霉)鏈熺殑 PCR 鐢�(ch菐n)鐗╅暦(zh菐ng)搴︾偤 560 bp銆�

3. PCR mix 涓惈涓婃ǎ鏌撴枡锛孭CR 鍚庡彲浠ョ洿鎺ヤ笂妯i浕娉炽€�

4. 鎻愪緵闄�(y谩ng)鎬у皪(du矛)鐓�锛屼究浜庡垎鏋愯│椹�(y脿n)绲�(ji茅)鏋�銆傛敞鎰忎簨闋�(xi脿ng)锛�

1锛庡熀绀�(ch菙)绋嬪簭锛�

2锛�?d膩ng)U澧炴韩搴﹀拰寤朵几婧害锛�

3锛庡弽鎳�(y墨ng)鏅�(sh铆)闁�锛�

4锛庡惊鐠�(hu谩n)娆℃暩(sh霉)锛�

5锛嶱CR 鍙嶆噳(y墨ng)娑茬殑閰嶅埗锛�

6锛嶱CR鎶€琛�(sh霉)鐨勫熀鏈師鐞�锛�

7锛嶱CR鐨勫弽鎳�(y墨ng)鍕�(d貌ng)鍔涘(xu茅)锛�

8锛嶱CR鎿�(ku貌)澧炵敘(ch菐n)鐗�锛�

9锛嶱CR鍙嶆噳(y墨ng)楂旂郴鑸囧弽鎳�(y墨ng)姊濅欢銆�

C1499669541542yqcpqd4b7upng_small (1).jpg

浣跨敤鍙婃晥鏋滐細

PCR鍙嶆噳(y墨ng)鐗归粸(di菐n)

(1) 鐗圭暟鎬у挤(qi谩ng)

PCR鍙嶆噳(y墨ng)鐨勭壒鐣版€ф焙瀹氬洜绱犵偤锛�

銆€銆€?銆佸儢類烽敱c妯℃澘DNA鐗圭暟姝g⒑鐨勭祼(ji茅)鍚堬紱

銆€銆€?銆佽冬A鍩洪厤灏�(du矛)鍘熷墖锛�

銆€銆€?銆佽房aq DNA鑱氬悎閰跺悎鎴愬弽鎳�(y墨ng)鐨勫繝瀵�(sh铆)鎬�锛�

銆€銆€銆€鈶i澏鍩哄洜鐨勭壒鐣版€ц垏淇濆畧鎬с€�

鍏朵腑寮曠墿鑸囨ā鏉跨殑姝g⒑绲�(ji茅)鍚堟槸闂�(gu膩n)閸点€傚紩鐗╄垏妯℃澘鐨勭祼(ji茅)鍚堝強寮曠墿閺堢殑寤朵几鏄伒寰牽鍩洪厤灏�(du矛)鍘熷墖鐨�銆傝仛鍚堥叾鍚堟垚鍙嶆噳(y墨ng)鐨勫繝瀵�(sh铆)鎬у強Taq DNA鑱氬悎閰惰€愰珮婧€�锛屼娇鍙嶆噳(y墨ng)涓ā鏉胯垏寮曠墿鐨勭祼(ji茅)鍚�(寰�(f霉)鎬�)鍙互鍦ㄨ純楂樼殑婧害涓嬮€�(j矛n)琛�锛岀祼(ji茅)鍚堢殑鐗圭暟鎬уぇ澶у鍔�锛岃鎿�(ku貌)澧炵殑闈跺熀鍥犵墖娈典篃灏辫兘淇濇寔寰堥珮鐨勬纰哄害銆傚啀閫氶亷(gu貌)閬告搰鐗圭暟鎬у拰淇濆畧鎬ч珮鐨勯澏鍩哄洜鍗€(q奴)锛屽叾鐗圭暟鎬х▼搴﹀氨鏇撮珮銆�

(2) 闈堟晱搴﹂珮

PCR鐢�(ch菐n)鐗╃殑鐢熸垚閲忔槸浠ユ寚鏁�(sh霉)鏂瑰紡澧炲姞鐨�锛岃兘灏囩毊鍏�(pg=10-12g)閲忕礆(j铆)鐨勮捣濮嬪緟娓�(c猫)妯℃澘鎿�(ku貌)澧炲埌寰厠(ug=10-6g)姘村钩銆傝兘寰�100钀�(w脿n)鍊�(g猫)绱�(x矛)鑳炰腑妾㈠嚭涓€鍊�(g猫)闈剁窗(x矛)鑳�锛涘湪鐥呮瘨鐨勬娓�(c猫)涓紝PCR鐨勯潏鏁忓害鍙仈(d谩)3鍊�(g猫)RFU(绌烘枒褰㈡垚鍠綅)锛涘湪绱�(x矛)鑿屽(xu茅)涓瓃ui灏忔鍑虹巼鐐�3鍊�(g猫)绱�(x矛)鑿屻€�

(3) 绨�(ji菐n)渚裤€佸揩閫�

PCR鍙嶆噳(y墨ng)鐢ㄨ€愰珮婧殑Taq DNA鑱氬悎閰讹紝涓€娆℃€у湴灏囧弽鎳�(y墨ng)娑插姞濂藉悗锛屽嵆鍦―NA鎿�(ku貌)澧炴恫鍜屾按娴撮崑涓婇€�(j矛n)琛岃畩鎬�-閫€鐏�-寤朵几鍙嶆噳(y墨ng)锛屼竴鑸湪2锝�4 灏忔檪(sh铆)瀹屾垚鎿�(ku貌)澧炲弽鎳�(y墨ng)銆傛摯(ku貌)澧炵敘(ch菐n)鐗╀竴鑸敤闆绘吵鍒嗘瀽锛屼笉涓€瀹氳鐢ㄥ悓浣嶇礌锛岀劇(w煤)鏀惧皠鎬ф薄鏌�銆佹槗鎺ㄥ唬銆�

(4) 灏�(du矛)妯�(bi膩o)鏈殑绱斿害瑕佹眰浣�

涓嶉渶瑕佸垎闆㈢梾姣掓垨绱�(x矛)鑿屽強鍩归(y菐ng)绱�(x矛)鑳�锛孌NA 绮楀埗鍝佸強绺絉NA鍧囧彲浣滅偤鎿�(ku貌)澧炴ā鏉�?锛熻妽鑻綉闂≧搴婃(bi膩o)鏈琛€娑层€侀珨鑵旀恫銆佹礂鍡芥恫銆佹瘺鐧�(f膩)銆佺窗(x矛)鑳炪€佹椿PCR鎶€琛�(sh霉)姒傝珫銆�



璨�(m菐i)瀹�鏁�(sh霉)閲�鎴愪氦鏅�(sh铆)闁�
姝e搧淇濋殰

纰轰繚鎵€鏈夌敘(ch菐n)鍝侀兘鏄師瑁濇鍝�

鍎�(y艒u)璩�(zh矛)鏈嶅嫏(w霉)

鍎�(y艒u)璩�(zh矛)鏈嶅嫏(w霉)锛屽敭鍚庣劇(w煤)鎲�

瀹夊叏鍖呰

绲�(t菕ng)涓€鍖呰锛屼繚闅滅敘(ch菐n)鍝佸畨鍏ㄩ亱(y霉n)杓�

姝h(gu墨)鐧�(f膩)绁�

姗�(j墨)鎵撶櫦(f膩)绁�锛岄檮绠遍€侀仈(d谩)

      鍟嗗搧椹�(y脿n)鏀�                绌嶅垎瑕�(gu墨)鍓�               閫€鎻涙鍦板潃            鎶曡ù寤鸿                鐧�(f膩)绁ㄥ埗搴�
      閰嶉€佹煡瑭�(x煤n)                璩�(g貌u)鐗╂祦绋�                閫€鎻涙娴佺▼            鑱�(li谩n)绯诲鏈�               浠樻鍛ㄦ湡
      閰嶉€佽(shu艒)鏄�                鏈�(hu矛)鍝¢珨绯�                閫€鎻涙鍘熷墖            鎵惧洖瀵嗙⒓               浠樻鏂瑰紡
涓�
涓�
涓�
涓�
涓�
鎵嬫(j墨)鎺冧竴鎺�
瑷晱(w猫n)鎵嬫(j墨)缍�(w菐ng)绔�