17-OHCS Elisa Kit鍘熺悊锛�
ELISA瑭﹀姂鐩掓槸鍥虹浉澶惧績娉曢叾鑱�(li谩n)鍏嶇柅鍚搁檮瀵﹂锛圗LISA锛�.宸茬煡寰呮脯鐗╄唱(zh矛)婵冨害鐨勬(bi膩o)婧�(zh菙n)鍝�銆佹湭鐭ユ績搴︾殑妯e搧鍔犲叆寰瓟閰舵(bi膩o)鏉垮収(n猫i)閫�(j矛n)琛屾娓�銆傚厛灏囧緟娓墿璩�(zh矛)鍜岀敓鐗╃礌妯�(bi膩o)瑷樼殑鎶楅珨鍚屾檪婧偛銆傛礂婊屽悗锛屽姞鍏ヨΚ鍜岀礌妯�(bi膩o)瑷橀亷鐨凥RP銆傚啀缍�(j墨ng)閬庢韩鑲插拰娲楁粚锛屽幓闄ゆ湭绲�(ji茅)鍚堢殑閰剁祼(ji茅)鍚堢墿锛岀劧鍚庡姞鍏ュ簳鐗〢銆丅锛屽拰閰剁祼(ji茅)鍚堢墿鍚屾檪浣滅敤銆傜敘(ch菐n)鐢熼鑹�銆傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑寰呮脯鐗╄唱(zh矛)鐨勬績搴﹀憟姣斾緥闂�(gu膩n)绯�銆�
17-OHCS Elisa Kit 绲勬垚锛�
搴忚櫉 | 鍚嶇ū | 瑕�(gu墨)鏍� |
1 | 閰舵(bi膩o)鍖呰鏉� | 96 瀛�*1 鍙媶鍗告澘 |
2 | 閰剁祼(ji茅)鍚堢墿 | 10ml*1 鐡� |
3 | 妯�(bi膩o)婧�(zh菙n)鍝� | 1ml*6 鐡� |
4 | 绶╂矕娑� | 6ml*1 鐡� |
5 | 椤壊鍔� A 娑� | 6ml*1 鐡� |
6 | 椤壊鍔� B 娑� | 6ml*1 鐡� |
7 | 绲傛娑� | 6ml*1 鐡� |
8 | 婵冪府娲楁粚娑诧紙*100锛� | 50ml*1 鐡� |
9 | 灏佹澘鑶� | 1 寮� |
10 | 浣跨敤瑾槑鏇� | 1 浠� |
鑷倷鏉愭枡
1锛夎捀椁炬按銆�
2锛夊姞妯e櫒锛�5ul銆�10ul銆�50ul銆�100ul銆�200ul銆�500ul銆�1000ul銆�
3锛夋尟钑╁櫒鍙婄鍔涙敧鎷屽櫒绛�銆�
17-OHCS Elisa Kit 妯�(bi膩o)鏈姹�
1.妯�(bi膩o)鏈噰闆嗗悗鐩℃棭閫�(j矛n)琛屾彁鍙�锛屾彁鍙栨寜鐩搁棞(gu膩n)鏂囩嵒(xi脿n)閫�(j矛n)琛岋紝鎻愬彇鍚庢噳(y墨ng)鐩″揩閫�(j矛n)琛屽椹�銆傝嫢涓嶈兘棣笂閫�(j矛n)琛岃│椹�锛屽彲灏囨(bi膩o)鏈斁浜�-20鈩冧繚瀛�锛屼絾鎳�(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺
2.涓嶈兘妾㈡脯鍚� NaN3 鐨勬ǎ鍝�锛屽洜 NaN3 鎶戝埗杈f牴閬庢哀鍖栫墿閰剁殑锛圚RP锛夋椿鎬�銆�
2.鎿嶄綔姝ラ
1.绶ㄨ櫉锛氬皣妯e搧灏嶆噳(y墨ng)寰瓟鎸夊簭绶ㄨ櫉锛屾瘡鏉挎噳(y墨ng)瑷�(sh猫)闄版€у皪鐓� 2 瀛斻€侀櫧鎬у皪鐓�2 瀛�銆佺┖鐧藉皪鐓� 1
瀛旓紙绌虹櫧灏嶇収瀛斾笉鍔犳ǎ鍝佸強閰舵(bi膩o)瑭﹀姂锛屽叾浣欏悇姝ユ搷浣滅浉鍚岋級
2.鍔犳ǎ锛氬垎鍒ュ湪闄�銆侀櫧鎬у皪鐓у瓟涓姞鍏ラ櫚鎬у皪鐓с€侀櫧鎬у皪鐓� 50渭l銆傜劧鍚庡湪寰呮脯妯e搧瀛斿厛鍔犳ǎ鍝佺█閲嬫恫 40渭l锛岀劧鍚庡啀鍔犲緟娓ǎ鍝� 10渭l銆傚姞妯e皣妯e搧鍔犱簬閰舵(bi膩o)鏉垮瓟搴曢儴锛岀洝閲忎笉瑙稿強瀛斿锛岃紩杓曟檭鍕曟贩鍕�锛�
3.婧偛锛氱敤灏佹澘鑶滃皝鏉垮悗缃� 37鈩冩韩鑲� 30 鍒嗛悩銆�
4.閰嶆恫锛氬皣 30 鍊嶆績绺礂婊屾恫鐢ㄨ捀椁炬按 30 鍊嶇█閲嬪悗鍌欑敤
5.娲楁粚锛氬皬蹇冩彮鎺夊皝鏉胯啘锛屾鍘绘恫楂�锛岀敥骞诧紝姣忓瓟鍔犳豢娲楁粚娑�锛岄潨缃� 30 绉掑悗妫勫幓锛屽姝ら噸寰�(f霉) 5 娆�锛屾媿骞层€�
6.鍔犻叾锛氭瘡瀛斿姞鍏ラ叾妯�(bi膩o)瑭﹀姂 50渭l锛岀┖鐧藉瓟闄ゅ銆�
7.婧偛锛氭搷浣滃悓 3銆�
8.娲楁粚锛氭搷浣滃悓 5銆�
9.椤壊锛氭瘡瀛斿厛鍔犲叆椤壊鍔� A50渭l锛屽啀鍔犲叆椤壊鍔� B50渭l锛岃紩杓曢渿钑╂贩鍕�锛�37鈩冮伩鍏夐’鑹�10 鍒嗛悩.
10.绲傛锛氭瘡瀛斿姞绲傛娑� 50渭l锛岀祩姝㈠弽鎳�(y墨ng)锛堟鏅傝棈(l谩n)鑹茬珛杞�(zhu菐n)榛冭壊锛夈€�
娓畾锛氫互绌虹櫧绌鸿(di脿o)闆�锛�450nm 娉㈤暦渚濆簭娓噺鍚勫瓟鐨勫惛鍏夊害锛圤D 鍊硷級銆� 娓畾鎳�(y墨ng)鍦ㄥ姞绲傛娑插悗 15 鍒嗛悩浠ュ収(n猫i)閫�(j矛n)琛�銆�
17-OHCS Elisa Kit 鏁�(sh霉)鎿�(j霉)瑷堢畻
1. 绻埗妯�(bi膩o)婧�(zh菙n)鏇茬窔锛氬悇妯�(bi膩o)婧�(zh菙n)鍝� OD 鍊兼笡鍘诲簳鑹插嵆娓涘幓绌虹櫧瀛� OD 鍊硷紝浠� 6 鍊嬫(bi膩o)婧�(zh菙n)
鍝佺殑 OD 鍊肩偤绺卞潗妯�(bi膩o) y锛屼互妯�(bi膩o)婧�(zh菙n)鍝佺殑婵冨害鐐烘┇鍧愭(bi膩o) x锛岀躬鍒舵洸绶氬湒锛屽鍦栫ず锛�
骞惰▓绠楀嚭鍥炴鏂圭▼ y=ax+b銆�
2. 灏囧緟娓ǎ鍝佺殑 OD 鍊间唬鍏ユ柟绋嬪紡锛岃▓绠楀悇绲勬ǎ鍝佹績搴�锛屽啀涔樹互绋€閲嬪€嶆暩(sh霉)锛屽嵆
鐐烘ǎ鍝佺殑瀵﹂殯 ABP 婵冨害銆�
3. 鏁忔劅搴︼細1.0 pg/ml
娉ㄦ剰浜嬮爡锛�
1. 瀵﹂鎿嶄綔涓繀闋堜娇鐢ㄤ竴娆℃€у惛闋紝閬垮厤浜ゅ弶姹℃煋銆�
2. 鍔犳ǎ锛氬姞妯f檪锛岃鎺у埗鍔犳ǎ閫熷害锛岄伩鍏�**瀛旇垏鏈€鍚庝竴瀛旈枔鐨勬檪闁撻枔闅旈亷澶�锛屽惁鍓囧皣鏈冨皫(d菐o)鑷翠笉鍚岀殑闋�(y霉)瀛佃偛鏅傞枔锛屽緸鑰屽奖闊�
瀵﹂鐨勬簴(zh菙n)纰烘€т互鍙婇噸寰�(f霉)鎬с€�
3. 瀛佃偛锛氬毚(y谩n)鏍兼寜鐓ц鏄庢浉涓婅(gu墨)瀹氱殑瀛佃偛鏅傞枔鍜屾韩搴﹂€�(j矛n)琛�銆�
4. 鍙嶆噳(y墨ng)鏅傞枔鐨勬帶鍒讹細鍔犲叆搴曠墿鍚庤珛瀹氭檪瑙€瀵熷弽鎳�(y墨ng)瀛旂殑椤忚壊璁婂寲锛屽鏋滈鑹茶純娣�锛岃珛?zh铆)宕嗗嚬灏よ櫌K姝㈡恫绲傛鍙嶆噳(y墨ng)銆�
5. 寤鸿瀵﹂鍓嶉爯(y霉)娓ǎ鍝佸惈閲�锛屽妯e搧婵冨害閬庨珮锛屾噳(y墨ng)灏嶆ǎ鍝侀€�(j矛n)琛岀█閲�锛岃▓绠楃祼(ji茅)鏋滄檪涔樹互鐩告噳(y墨ng)鐨勭█閲嬪€嶆暩(sh霉)銆�
6. 寤鸿浣跨敤鏈│鍔戠洅鏅傚厛鍋氶爯(y霉)瀵﹂锛堝嵆鍏堝仛妯�(bi膩o)婧�(zh菙n)鏇茬窔锛岃│鐢ㄥ咕鍊嬫(bi膩o)鏈級锛屽鏋滃皪鏈│鍔戠洅鏈変换浣曠枒鍟�锛屽彲鍜屾墍璩肩稉(j墨ng)閵峰晢鑱�(li谩n)绯�锛�
濡傛灉鍥犻亱杓搁亷绋嬪皫(d菐o)鑷磋│鍔戠洅澶辨晥锛屽彲瑕佹眰瑾�(di脿o)鎻�锛屼絾姒備笉鎵挎摂(d膩n)鐢�(ch菐n)鍝佹湰韬互澶栫殑浠讳綍鎼嶅け銆�
17-OHCS Elisa Kit瀹夊叏鎬�
1锛� 閬垮厤浜洪珨鐩存帴鎺ヨЦ椤壊鍔慉銆丅鍜岀祩姝㈡恫銆備竴鏃︽帴瑙稿埌閫欎簺娑查珨锛岃珛鐩″揩鐢ㄦ按娌栨礂銆�
2锛� 瀵﹂涓笉瑕侀€�(j矛n)椋�銆佹娊鐓欐垨浣跨敤鍖栧鍝併€�
3锛� 涓嶈鐢ㄥ槾鍚稿彇瑭﹀姂鐩掗噷鐨勪换浣曟垚浠�銆�
淇濆瓨姊濅欢鍙婃湁鏁堟湡锛�
1.瑭﹀姂鐩掍繚瀛橈細2-8鈩�
2.鏈夋晥鏈燂細6鍊嬫湀